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pLVX-Tight-Puro-KrasG12D2B
(Plasmid #139786)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139786 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX-Tight-Puro
  • Backbone size w/o insert (bp) 7791
  • Total vector size (bp) 8358
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    KrasG12D
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    567
  • Mutation
    G12D
  • Entrez Gene
    Kras (a.k.a. K-Ras, K-Ras 2, K-ras, Ki-ras, Kras-2, Kras2, c-K-ras, c-Ki-ras, p21B, ras)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-Tight-Puro-KrasG12D2B was a gift from Joan Massague (Addgene plasmid # 139786 ; http://n2t.net/addgene:139786 ; RRID:Addgene_139786)

  • For your References section:

    TGF-beta orchestrates fibrogenic and developmental EMTs via the RAS effector RREB1. Su J, Morgani SM, David CJ, Wang Q, Er EE, Huang YH, Basnet H, Zou Y, Shu W, Soni RK, Hendrickson RC, Hadjantonakis AK, Massague J. Nature. 2020 Jan;577(7791):566-571. doi: 10.1038/s41586-019-1897-5. Epub 2020 Jan 8. 10.1038/s41586-019-1897-5 PubMed 31915377
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