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mCherry-Myo10-∆PH
(Plasmid #139781)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139781 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-Myo10
  • Backbone manufacturer
    Richard E. Cheney, UNC
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Myosin X
  • Alt name
    MYO10
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5154
  • Mutation
    Deletion of 3PH domains
  • Entrez Gene
    MYO10 (a.k.a. MyoX)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BgIII (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-Myo10-∆PH was a gift from Staffan Stromblad (Addgene plasmid # 139781 ; http://n2t.net/addgene:139781 ; RRID:Addgene_139781)

  • For your References section:

    PtdIns(3,4,5)P(3) is a regulator of myosin-X localization and filopodia formation. Plantard L, Arjonen A, Lock JG, Nurani G, Ivaska J, Stromblad S. J Cell Sci. 2010 Oct 15;123(Pt 20):3525-34. doi: 10.1242/jcs.069609. 10.1242/jcs.069609 PubMed 20930142
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