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RCAS (A) Wnt3a (CT#169)
(Plasmid #13976)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13976 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RCASBP(A)
  • Backbone size w/o insert (bp) 11600
  • Vector type
    Retroviral ; avian expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Wnt3a
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Wnt3a (a.k.a. Wnt-3a, vt)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer RCAS-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A mouse Wnt3a cDNA encoding the entire open reading frame was cloned into the shuttle vector SLAX-13 and then subloned into the RCAS retroviral vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS (A) Wnt3a (CT#169) was a gift from Cliff Tabin (Addgene plasmid # 13976 ; http://n2t.net/addgene:13976 ; RRID:Addgene_13976)

  • For your References section:

    Distinct WNT pathways regulating AER formation and dorsoventral polarity in the chick limb bud. Kengaku M, Capdevila J, Rodriguez-Esteban C, De La Pena J, Johnson RL, Belmonte JC, Tabin CJ. Science. 1998 May 22. 280(5367):1274-7. 10.1126/science.280.5367.1274 PubMed 9596583
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