TBXTA-c021
(Plasmid
#139758)
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PurposeProtein expression/crystallography (PDB:5QT0)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139758 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSUMO-LIC
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Backbone manufacturerSGC
- Backbone size w/o insert (bp) 5610
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTBXT, DNA-binding domain, G177D
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Alt nameBrachyury
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SpeciesH. sapiens (human)
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Insert Size (bp)554
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MutationG177D; contains only amino acids E41-N211
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Entrez GeneTBXT (a.k.a. SAVA, T, TFT)
- Promoter T7
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Tag
/ Fusion Protein
- His6-SUMO (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
E41-N211 : Codon-optimized for E. coli expression
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TBXTA-c021 was a gift from Opher Gileadi (Addgene plasmid # 139758 ; http://n2t.net/addgene:139758 ; RRID:Addgene_139758)
