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Addgene

TBXTA-c006
(Plasmid #139755)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139755 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Merck
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TBXT, DNA-binding domain, G177D
  • Alt name
    Brachyury
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    590
  • Mutation
    G177D; contains only amino acids E41-S224
  • Entrez Gene
    TBXT (a.k.a. SAVA, T, TFT)
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7F
  • 3′ sequencing primer T7R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

E41-S224 : Codon-optimized for E. coli expression

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TBXTA-c006 was a gift from Opher Gileadi (Addgene plasmid # 139755 ; http://n2t.net/addgene:139755 ; RRID:Addgene_139755)
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