pGEM cWnt10a (CT#588)
(Plasmid
#13973)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13973 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWnt10a
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SpeciesG. gallus (chicken)
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Insert Size (bp)400
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Entrez GeneWNT10A
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDevyn Smith
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
400 bp fragment of cWnt10a generated by degenerate PCR, for in situ.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEM cWnt10a (CT#588) was a gift from Cliff Tabin (Addgene plasmid # 13973 ; http://n2t.net/addgene:13973 ; RRID:Addgene_13973) -
For your References section:
Wnt signaling during development of the gastrointestinal tract. Theodosiou NA, Tabin CJ. Dev Biol. 2003 Jul 15. 259(2):258-71. 10.1016/S0012-1606(03)00185-4 PubMed 12871700