RCAS(A) cTbx5 VP16 (CT#630)
(Plasmid
#13969)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13969 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneRCASBP(A)
- Backbone size w/o insert (bp) 11600
-
Vector typeRetroviral ; avian expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameTbx5
-
SpeciesG. gallus (chicken)
-
MutationAmino acids 1-274
-
Entrez GeneTBX5
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer RCAS-F (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains amino acids 1-274 of Tbx5 fused to a duplex of the lambda hinge region and VP16 (transcriptional activator).
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
RCAS(A) cTbx5 VP16 (CT#630) was a gift from Cliff Tabin (Addgene plasmid # 13969 ; http://n2t.net/addgene:13969 ; RRID:Addgene_13969) -
For your References section:
Tbx5 is required for forelimb bud formation and continued outgrowth. Rallis C, Bruneau BG, Del Buono J, Seidman CE, Seidman JG, Nissim S, Tabin CJ, Logan MP. Development. 2003 Jun . 130(12):2741-51. 10.1242/dev.00473 PubMed 12736217