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pcDNA8-Flag-LARS(1-976aa)
(Plasmid #139689)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139689 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3-FLAG TAGGED
  • Backbone manufacturer
    Flag inserted and cloning site modified in lab
  • Backbone size w/o insert (bp) 5400
  • Modifications to backbone
    CMV promoter - T7 promoter - HindI - NotI - Kozak sequence - Flag - BamHI - LRS-XhoI…
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LARS1
  • Alt name
    Leucyl-tRNA-synthetase
  • Alt name
    LRS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2928
  • Entrez Gene
    LARS1 (a.k.a. HSPC192, ILFS1, LARS, LEURS, LEUS, LFIS, LRS, PIG44, RNTLS, cLRS, hr025Cl)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-REV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA8-Flag-LARS(1-976aa) was a gift from Jie Chen (Addgene plasmid # 139689 ; http://n2t.net/addgene:139689 ; RRID:Addgene_139689)

  • For your References section:

    Leucyl-tRNA Synthetase Activates Vps34 in Amino Acid-Sensing mTORC1 Signaling. Yoon MS, Son K, Arauz E, Han JM, Kim S, Chen J. Cell Rep. 2016 Aug 9;16(6):1510-1517. doi: 10.1016/j.celrep.2016.07.008. Epub 2016 Jul 28. 10.1016/j.celrep.2016.07.008 PubMed 27477288
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