pMT-Hrp48-ADARcd-E488Q-V5
(Plasmid
#139685)
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PurposeExpresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assays
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 139685 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMT
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Vector typeBacterial Expression, Insect Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHrp48, ADAR
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SpeciesD. melanogaster (fly)
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Entrez GeneAdar (a.k.a. Dmel_CG12598, ADAR, ADAR1, CG12598, Dmel\CG12598, EG:BACN35H14.1, adar, adr, cg12598, dADAR, dAdar, dadar, hypnos-2)
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMT-Hrp48-ADARcd-E488Q-V5 was a gift from Michael Rosbash (Addgene plasmid # 139685 ; http://n2t.net/addgene:139685 ; RRID:Addgene_139685) -
For your References section:
Mechanistic implications of enhanced editing by a HyperTRIBE RNA-binding protein. Xu W, Rahman R, Rosbash M. RNA. 2018 Feb;24(2):173-182. doi: 10.1261/rna.064691.117. Epub 2017 Nov 10. 10.1261/rna.064691.117 PubMed 29127211