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Addgene

pLVX Nup62Fv2GFP
(Plasmid #139683)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139683 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 8000
  • Total vector size (bp) 11455
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nup62
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3342
  • GenBank ID
    BC003663.2 23636
  • Entrez Gene
    NUP62 (a.k.a. IBSN, SNDI, p62)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Dimerization domain FKBP (C terminal on insert)
    • 2 copies of GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site BstB1 (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer PGK rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX Nup62Fv2GFP was a gift from Edward Campbell (Addgene plasmid # 139683 ; http://n2t.net/addgene:139683 ; RRID:Addgene_139683)

  • For your References section:

    Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus. Dharan A, Bachmann N, Talley S, Zwikelmaier V, Campbell EM. Nat Microbiol. 2020 Jun 1. pii: 10.1038/s41564-020-0735-8. doi: 10.1038/s41564-020-0735-8. 10.1038/s41564-020-0735-8 PubMed 32483230
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