RCAS(A) cTbx5 EnR (CT#635)
(Plasmid
#13967)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13967 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneRCASBP(A)
- Backbone size w/o insert (bp) 11600
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Vector typeRetroviral ; avian expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTbx5
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SpeciesG. gallus (chicken)
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MutationAmino acids 1-274
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Entrez GeneTBX5
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer RCAS-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains amino acids 1-274 of the full-length Tbx5 clone fused to amino acids 2-298 of the Drosophila Engrailed protein.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RCAS(A) cTbx5 EnR (CT#635) was a gift from Cliff Tabin (Addgene plasmid # 13967 ; http://n2t.net/addgene:13967 ; RRID:Addgene_13967) -
For your References section:
Tbx5 is required for forelimb bud formation and continued outgrowth. Rallis C, Bruneau BG, Del Buono J, Seidman CE, Seidman JG, Nissim S, Tabin CJ, Logan MP. Development. 2003 Jun . 130(12):2741-51. 10.1242/dev.00473 PubMed 12736217