Skip to main content
Addgene

pcDNA8-Flag-LARS(1-360aa)
(Plasmid #139668)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139668 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3-Flag
  • Backbone manufacturer
    modified by Jie Chen lab
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 6500
  • Modifications to backbone
    CMVpromoter-T7promoter HindI-NotI-kozak sequence-Flag-BamHI-INSERT-XhoI
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Leucyl-tRNA Synthetase
  • Alt name
    LARS1
  • Alt name
    LRS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1080
  • GenBank ID
    NM_020117.11
  • Entrez Gene
    LARS1 (a.k.a. HSPC192, ILFS1, LARS, LEURS, LEUS, LFIS, LRS, PIG44, RNTLS, cLRS, hr025Cl)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV foward
  • 3′ sequencing primer Bgh-Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA8-Flag-LARS(1-360aa) was a gift from Jie Chen (Addgene plasmid # 139668 ; http://n2t.net/addgene:139668 ; RRID:Addgene_139668)

  • For your References section:

    Leucyl-tRNA Synthetase Activates Vps34 in Amino Acid-Sensing mTORC1 Signaling. Yoon MS, Son K, Arauz E, Han JM, Kim S, Chen J. Cell Rep. 2016 Aug 9;16(6):1510-1517. doi: 10.1016/j.celrep.2016.07.008. Epub 2016 Jul 28. 10.1016/j.celrep.2016.07.008 PubMed 27477288
Related items:
Commonly requested with: