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pET303C-hSOD1 A4V/C6S
(Plasmid #139667)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139667 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET303C
  • Backbone manufacturer
    N/A
  • Backbone size w/o insert (bp) 5363
  • Total vector size (bp) 5828
  • Modifications to backbone
    N/A
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Superoxide dismutase-1
  • Alt name
    SOD1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    465
  • Mutation
    change alanine 4 to valine, change cysteine 6 to serine
  • Entrez Gene
    SOD1 (a.k.a. ALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    GenScript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET303C-hSOD1 A4V/C6S was a gift from Svetlana Antonyuk (Addgene plasmid # 139667 ; http://n2t.net/addgene:139667 ; RRID:Addgene_139667)

  • For your References section:

    Ebselen as template for stabilization of A4V mutant dimer for motor neuron disease therapy. Chantadul V, Wright GSA, Amporndanai K, Shahid M, Antonyuk SV, Washbourn G, Rogers M, Roberts N, Pye M, O'Neill PM, Hasnain SS. Commun Biol. 2020 Mar 5;3(1):97. doi: 10.1038/s42003-020-0826-3. 10.1038/s42003-020-0826-3 PubMed 32139772
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