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Addgene

pLL3.7-K122-FH-TAZ-TEAD-p-H2B-mCherry
(Plasmid #139649)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139649 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLL3.7
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B-mCherry
  • Alt name
    TAZ
  • Alt name
    IRES-GFP
  • Tag / Fusion Protein
    • FLAG, His6 (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

H2B-mCherry is derived from Addgene

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLL3.7-K122-FH-TAZ-TEAD-p-H2B-mCherry was a gift from Yutaka Hata (Addgene plasmid # 139649 ; http://n2t.net/addgene:139649 ; RRID:Addgene_139649)

  • For your References section:

    A cell-based screening for TAZ activators identifies ethacridine, a widely used antiseptic and abortifacient, as a compound that promotes dephosphorylation of TAZ and inhibits adipogenesis in C3H10T1/2 cells. Kawano S, Maruyama J, Nagashima S, Inami K, Qiu W, Iwasa H, Nakagawa K, Ishigami-Yuasa M, Kagechika H, Nishina H, Hata Y. J Biochem. 2015 May 14. pii: mvv051. 10.1093/jb/mvv051 PubMed 25979969
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