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Addgene

pS241
(Plasmid #139645)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139645 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSL1190
  • Backbone size w/o insert (bp) 3422
  • Total vector size (bp) 5072
  • Vector type
    Bacterial Expression ; Cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ced-4
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    1650
  • Mutation
    addition of K212 and R213 compared to NCBI reference: NP_001021203.1
  • GenBank ID
    NM_001026031.2
  • Entrez Gene
    ced-4 (a.k.a. CELE_C35D10.9)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sma I (unknown if destroyed)
  • 3′ cloning site Spe I (unknown if destroyed)
  • 5′ sequencing primer M13 Fw
  • 3′ sequencing primer M13 Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pS241 was a gift from Robert Horvitz (Addgene plasmid # 139645 ; http://n2t.net/addgene:139645 ; RRID:Addgene_139645)

  • For your References section:

    An alternatively spliced C. elegans ced-4 RNA encodes a novel cell death inhibitor. Shaham S, Horvitz HR. Cell. 1996 Jul 26;86(2):201-8. doi: 10.1016/s0092-8674(00)80092-6. 10.1016/s0092-8674(00)80092-6 PubMed 8706125
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