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Addgene

phR-Luc
(Plasmid #139644)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139644 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL4.73
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Renilla luciferase
  • Species
    H. sapiens (human)
  • Promoter SV40

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer primers designed based on sequences
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' Cloning Site: 5' CTAGCAAAATAGGCTGTCCC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    phR-Luc was a gift from Markku Varjosalo (Addgene plasmid # 139644 ; http://n2t.net/addgene:139644 ; RRID:Addgene_139644)

  • For your References section:

    PTPRA Phosphatase Regulates GDNF-Dependent RET Signaling and Inhibits the RET Mutant MEN2A Oncogenic Potential. Yadav L, Pietila E, Ohman T, Liu X, Mahato AK, Sidorova Y, Lehti K, Saarma M, Varjosalo M. iScience. 2020 Feb 21;23(2):100871. doi: 10.1016/j.isci.2020.100871. Epub 2020 Jan 31. 10.1016/j.isci.2020.100871 PubMed 32062451
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