pcDNA3.1-Y1214F-VEGFR2-mCherry
(Plasmid #139609)

• Purpose: Expresses a mutant (Y1214F) VEGFR2-mCherry fusion protein in mammalian cells
• Depositing Lab: Michael Welsh
• Publication: Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121645. doi: 10.3390/cells8121645.

Backbone

• Vector backbone: pcDNA3.1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: KDR
• Species: H. sapiens (human)
• Insert Size (bp): 4078
• Entrez Gene: KDR (a.k.a. CD309, FLK1, VEGFR, VEGFR2)
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please Note: The translated product will have three extra amino acids that will be cleaved off as a signal sequence during membrane insertion.

Please See:
"VEGF receptor-2 Y951 signaling and a role for the adapter molecule TSAd in tumor angiogenesis."
Matsumoto T, Bohman S, Dixelius J, Berge T, Dimberg A, Magnusson P, Wang L, Wikner C, Qi JH, Wernstedt C, Wu J, Bruheim S, Mugishima H, Mukhopadhyay D, Spurkland A, Claesson-Welsh L.
EMBO J. 2005 Jul 6;24(13):2342-53. doi: 10.1038/sj.emboj.7600709. Epub 2005 Jun 16.
PMID: 15962004