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PurposeExpresses a VEGFR2-mCherry fusion protein in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 139606 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameKDR
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SpeciesH. sapiens (human)
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Insert Size (bp)4078
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Entrez GeneKDR (a.k.a. CD309, FLK1, VEGFR, VEGFR2)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-VEGFR2-mCherry was a gift from Michael Welsh (Addgene plasmid # 139606 ; http://n2t.net/addgene:139606 ; RRID:Addgene_139606) -
For your References section:
Temporal Dynamics of VEGFA-Induced VEGFR2/FAK Co-Localization Depend on SHB. Pietila I, Van Mourik D, Tamelander A, Kriz V, Claesson-Welsh L, Tengholm A, Welsh M. Cells. 2019 Dec 15;8(12). pii: cells8121645. doi: 10.3390/cells8121645. 10.3390/cells8121645 PubMed 31847469