pBS mPax2 (CT#670)
(Plasmid
#13960)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13960 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBS KS+
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePax2
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SpeciesM. musculus (mouse)
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MutationThis is not full lenght Pax2. There is a 100 nuncleotide truncation at the 3' end of the insert.
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Entrez GenePax2 (a.k.a. Opdc, Pax-2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byRichard Maas
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The Tabin lab only used this clone for in situ hybridization. Pax2 activity was never tested.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS mPax2 (CT#670) was a gift from Cliff Tabin (Addgene plasmid # 13960 ; http://n2t.net/addgene:13960 ; RRID:Addgene_13960) -
For your References section:
Dach1, a vertebrate homologue of Drosophila dachshund, is expressed in the developing eye and ear of both chick and mouse and is regulated independently of Pax and Eya genes. Heanue TA, Davis RJ, Rowitch DH, Kispert A, McMahon AP, Mardon G, Tabin CJ. Mech Dev. 2002 Feb . 111(1-2):75-87. 10.1016/S0925-4773(01)00611-6 PubMed 11804780