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Addgene

pET28a-AlpJ
(Plasmid #139598)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139598 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5308
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    alpJ
  • Species
    Streptomyces ambofaciens ATCC 23877
  • Promoter T7
  • Tag / Fusion Protein
    • N terminal hexahistidine (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-AlpJ was a gift from Emily Balskus (Addgene plasmid # 139598 ; http://n2t.net/addgene:139598 ; RRID:Addgene_139598)

  • For your References section:

    Production of Stealthin C Involves an S-N-Type Smiles Rearrangement. Wang P, Hong GJ, Wilson MR, Balskus EP. J Am Chem Soc. 2017 Mar 1;139(8):2864-2867. doi: 10.1021/jacs.6b10586. Epub 2017 Feb 16. 10.1021/jacs.6b10586 PubMed 28191843
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