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Addgene

pET28a-KinL
(Plasmid #139595)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139595 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5308
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    kinL
  • Species
    Streptomyces murayamaensis ATCC 21414
  • Insert Size (bp)
    1504
  • Promoter T7
  • Tag / Fusion Protein
    • hexahistidine (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-KinL was a gift from Emily Balskus (Addgene plasmid # 139595 ; http://n2t.net/addgene:139595 ; RRID:Addgene_139595)

  • For your References section:

    Glutamic acid is a carrier for hydrazine during the biosyntheses of fosfazinomycin and kinamycin. Wang KA, Ng TL, Wang P, Huang Z, Balskus EP, van der Donk WA. Nat Commun. 2018 Sep 11;9(1):3687. doi: 10.1038/s41467-018-06083-7. 10.1038/s41467-018-06083-7 PubMed 30206228
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