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Addgene

pET28a D315A
(Plasmid #139592)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139592 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    peT28a
  • Backbone manufacturer
    5308
  • Total vector size (bp) 6724
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SznF D315A
  • Insert Size (bp)
    1416
  • Mutation
    changed apartate 315 to alanine
  • Promoter T7
  • Tag / Fusion Protein
    • hexahistidine (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a D315A was a gift from Emily Balskus (Addgene plasmid # 139592 ; http://n2t.net/addgene:139592 ; RRID:Addgene_139592)

  • For your References section:

    An N-nitrosating metalloenzyme constructs the pharmacophore of streptozotocin. Ng TL, Rohac R, Mitchell AJ, Boal AK, Balskus EP. Nature. 2019 Feb;566(7742):94-99. doi: 10.1038/s41586-019-0894-z. Epub 2019 Feb 6. 10.1038/s41586-019-0894-z PubMed 30728519
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