pBS cDach1 (CT#319)
(Plasmid
#13959)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13959 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBS SK+
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDachshund1
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Alt nameDach1
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SpeciesG. gallus (chicken)
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Insert Size (bp)3000
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Entrez GeneDACH1 (a.k.a. DACH)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For in situ probe, lacks the 5' end.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS cDach1 (CT#319) was a gift from Cliff Tabin (Addgene plasmid # 13959 ; http://n2t.net/addgene:13959 ; RRID:Addgene_13959) -
For your References section:
Dach1, a vertebrate homologue of Drosophila dachshund, is expressed in the developing eye and ear of both chick and mouse and is regulated independently of Pax and Eya genes. Heanue TA, Davis RJ, Rowitch DH, Kispert A, McMahon AP, Mardon G, Tabin CJ. Mech Dev. 2002 Feb . 111(1-2):75-87. 10.1016/S0925-4773(01)00611-6 PubMed 11804780