pBS cScx 3'UTR (CT#485)
(Plasmid
#13957)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13957 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBS SK
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameScleraxis
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Alt nameScx
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SpeciesG. gallus (chicken)
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Insert Size (bp)580
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Entrez GeneSCX (a.k.a. SCXB)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAndrew Lassar
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
cScx digested by EcoRV, Bgl II and religated; ~580bp (80 bp of coding plus 500 bp of UTR).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS cScx 3'UTR (CT#485) was a gift from Cliff Tabin (Addgene plasmid # 13957 ; http://n2t.net/addgene:13957 ; RRID:Addgene_13957) -
For your References section:
Analysis of the tendon cell fate using Scleraxis, a specific marker for tendons and ligaments. Schweitzer R, Chyung JH, Murtaugh LC, Brent AE, Rosen V, Olson EN, Lassar A, Tabin CJ. Development. 2001 Oct . 128(19):3855-66. PubMed 11585810