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Addgene

PB-TET-ZH-lox66FRT
(Plasmid #139563)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139563 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    PB-TET (addgene #20909)
  • Backbone manufacturer
    Andras Nagy (Addgene plasmid # 20909)
  • Backbone size w/o insert (bp) 12956
  • Vector type
    Mammalian Expression ; piggyBac
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PGK-ZeoR-pA cassette, inserted in PB-TET vector
  • Species
    Synthetic

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-TET-ZH-lox66FRT was a gift from Minoru Ko (Addgene plasmid # 139563 ; http://n2t.net/addgene:139563 ; RRID:Addgene_139563)