PB-TET-BH-lox66FRT
(Plasmid
#139562)
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PurposepiggyBac expression vector with TET-ON system and Blasticidin selection
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 139562 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePB-TET (addgene #20909)
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Backbone manufacturerAndras Nagy (Addgene plasmid # 20909)
- Backbone size w/o insert (bp) 12702
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Vector typeMammalian Expression ; piggyBac
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePGK-BSD-pA cassette, inserted in PB-TET vector
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SpeciesSynthetic
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-TET-BH-lox66FRT was a gift from Minoru Ko (Addgene plasmid # 139562 ; http://n2t.net/addgene:139562 ; RRID:Addgene_139562)