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pBS cSugarCrisp (CT#708)
(Plasmid #13956)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13956 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBS
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SugarCrisp
  • Alt name
    Pi15
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    1000
  • Entrez Gene
    PI15

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SugarCrisp cDNA ~1kb Cla I fragment derived from pMIW/Sugar Crisp.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS cSugarCrisp (CT#708) was a gift from Cliff Tabin (Addgene plasmid # 13956 ; http://n2t.net/addgene:13956 ; RRID:Addgene_13956)

  • For your References section:

    Cloning and expression of a novel cysteine-rich secreted protein family member expressed in thyroid and pancreatic mesoderm within the chicken embryo. Smith DM, Collins-Racie LA, Marigo VA, Roberts DJ, Davis NM, Hartmann C, Schweitzer R, LaVallie ER, Gamer L, McCoy J, Tabin CJ. Mech Dev. 2001 Apr . 102(1-2):223-6. 10.1016/S0925-4773(01)00293-3 PubMed 11287197
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