pBS cGli3 (CT#34)
(Plasmid
#13953)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13953 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBS SK
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGli3
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Alt namep19a1.5
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SpeciesG. gallus (chicken)
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Insert Size (bp)1500
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Entrez GeneGLI3 (a.k.a. GLI3FL)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For in situ probe; 3’ EcoRI fragment of cGli3 including the stop, does not contain the zinc finger region (denoted 19a1.5).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS cGli3 (CT#34) was a gift from Cliff Tabin (Addgene plasmid # 13953 ; http://n2t.net/addgene:13953 ; RRID:Addgene_13953) -
For your References section:
Similar expression and regulation of Gli2 and Gli3 in the chick limb bud. Schweitzer R, Vogan KJ, Tabin CJ. Mech Dev. 2000 Nov . 98(1-2):171-4. 10.1016/S0925-4773(00)00458-5 PubMed 11044624