pDEST-R4R3-MC1DTA
(Plasmid
#139521)
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Purpose(Empty Backbone) Provides negative selection cassette on the vector
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139521 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMC1DT-ApA
- Backbone size (bp) 4338
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Vector typeMouse Targeting
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Selectable markersDiphtheria toxin A
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ccdB-Cm cassette was inserted on the backbone; Uses Diphtheria toxin A (DTA) as negative selection.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST-R4R3-MC1DTA was a gift from Minoru Ko (Addgene plasmid # 139521 ; http://n2t.net/addgene:139521 ; RRID:Addgene_139521)
