pDEST-EB-Hyg-R4R3
(Plasmid
#139520)
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Purpose(Empty Backbone) Provides episomal replication of the destination vector in primate cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139520 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEB-C5 (Addgene #28213)
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Backbone manufacturerLinzhao Cheng (Addgene plasmid # 28213)
- Backbone size (bp) 11319
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ccdB-Cm cassette was inserted on the backbone
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST-EB-Hyg-R4R3 was a gift from Minoru Ko (Addgene plasmid # 139520 ; http://n2t.net/addgene:139520 ; RRID:Addgene_139520)
