pDEST-PB-R4R3
(Plasmid
#139518)
-
Purpose(Empty Backbone) Provides two PiggyBac transposon sequences between gene cassettes in the destination vector
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 139518 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonePB-TET (addgene #20909)
-
Backbone manufacturerAndras Nagy (Addgene plasmid # 20909)
- Backbone size (bp) 6112
-
Vector typeMammalian Expression ; piggyBac
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)ccdB Survival
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNone
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ccdB-Cm cassette was inserted between PB sequences
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pDEST-PB-R4R3 was a gift from Minoru Ko (Addgene plasmid # 139518 ; http://n2t.net/addgene:139518 ; RRID:Addgene_139518)