pSP571
(Plasmid #139498)

• Purpose: CRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNA
• Depositing Lab: Serge Pelet
• Publication: Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi: 10.1038/s41467-020-16943-w.

Backbone

• Vector backbone: pRS423II
• Backbone size w/o insert (bp): 5726
• Vector type: Yeast Expression
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pGPD Cas9 / sgRNA (STL162)
• gRNA/shRNA sequence: ATAAGCAGAACCAGTCACTGGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCT
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 4413
• Mutation: WT
• Promoter: pGPD

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpHI (unknown if destroyed)
• 3′ cloning site: SacII (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• Supplemental Documents:
  • pSP571_pRS423_Cas9_sgRNA_STL1.gb
• A portion of this plasmid was derived from a plasmid made by: Modified from Addgene #35464
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry