pISH-Olfr156
(Plasmid
#139486)
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PurposeTemplate to synthesize riboprobes for in situ hybridization (ISH)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 139486 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEM-T-easy
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 3300
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Modifications to backboneInsert of probe sequence
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Vector typeISH probe
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameISH probe for Olfr156 - UTR
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Alt namepISH-Olfr156
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SpeciesM. musculus (mouse)
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Insert Size (bp)348
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Entrez GeneOlfr156 (a.k.a. MOR262-6, OR37B, Olfr37b, mOR37b)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13f
- 3′ sequencing primer M13r (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pISH-Olfr156 was a gift from Peter Mombaerts (Addgene plasmid # 139486 ; http://n2t.net/addgene:139486 ; RRID:Addgene_139486) -
For your References section:
The Zonal Organization of Odorant Receptor Gene Choice in the Main Olfactory Epithelium of the Mouse. Zapiec B, Mombaerts P. Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 10.1016/j.celrep.2020.02.110. 10.1016/j.celrep.2020.02.110 PubMed 32209480