pCSIIneo-TRE-hPhyB621- mCherry-HRasCT
(Plasmid #139482)

• Purpose: Expresses PhyB621-mCherry-HRasCT in mammalian cells.
• Depositing Lab: Kazuhiro Aoki
• Publication: Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2906. doi: 10.1021/acschembio.0c00477. Epub 2020 Nov 9.

Backbone

• Vector backbone: pCSIIneo-TRE
• Backbone size w/o insert (bp): 9668
• Total vector size (bp): 12323
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PhyB621
• Alt name: phytochrome b
• Species: A. thaliana (mustard weed)
• Mutation: G229L and deletion of aa Y at position 235 in mcherry
• Entrez Gene: PHYB (a.k.a. AT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B)
• Promoter: Tet responsible element
• Tag / Fusion Protein:
  • mCherry-HRasCT (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: not determined
• 3′ sequencing primer: GGGCCCTCACATTGCCAAA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Depositor confirmed that mutation G229L and deletion of amino acid Y at position 235 in mCherry has no functional effect.