pT2Apuro-TRE-synPCB2.0
(Plasmid #139481)

• Purpose: Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.
• Depositing Lab: Kazuhiro Aoki
• Publication: Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2906. doi: 10.1021/acschembio.0c00477. Epub 2020 Nov 9.

Backbone

• Vector backbone: pT2Apuro-TRE
• Backbone size w/o insert (bp): 5208
• Total vector size (bp): 8379
• Vector type: Mammalian Expression ; Tol2 transposon donor vector
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
• Alt name: synPCB2.0
• Species: Synthetic; Thermosynechococcus elongatus bp-1
• Insert Size (bp): 3171
• Promoter: Tet responsive element

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: not determined
• 3′ sequencing primer: GGGCCCTCACATTGCCAAA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry