pCSIIhyg-rtTA
(Plasmid
#139480)
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PurposeExpresses rtTA, Tet-ON reverse tetracycline-controlled trans-activator in mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139480 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCSIIhyg
- Backbone size w/o insert (bp) 10830
- Total vector size (bp) 11577
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Vector typeMammalian Expression, Lentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namertTA, Tet-ON reverse transactivator
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SpeciesSynthetic
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Insert Size (bp)747
- Promoter Human EF1a promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer ACACTGAGTGGGTGGAGACTG
- 3′ sequencing primer GGGCCCTCACATTGCCAAA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCSIIhyg-rtTA was a gift from Kazuhiro Aoki (Addgene plasmid # 139480 ; http://n2t.net/addgene:139480 ; RRID:Addgene_139480) -
For your References section:
Improvement of Phycocyanobilin Synthesis for Genetically Encoded Phytochrome-Based Optogenetics. Uda Y, Miura H, Goto Y, Yamamoto K, Mii Y, Kondo Y, Takada S, Aoki K. ACS Chem Biol. 2020 Nov 20;15(11):2896-2906. doi: 10.1021/acschembio.0c00477. Epub 2020 Nov 9. 10.1021/acschembio.0c00477 PubMed 33164485
