p_SNIPR_target_GUmut33AtoG
(Plasmid
#139468)
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PurposeT7 RNAP-driven expression of a non-cognate SNIPR trigger RNA with a mutation at position 33
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139468 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET15b
- Backbone size w/o insert (bp) 5527
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTarget_mutGU33
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SpeciesOther
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Insert Size (bp)129
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CCTGCCACCATACCCACGC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Non-cognate target for the SNIPR with a mismatch at base 33 leading to a G-U base pair with SNIPR
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p_SNIPR_target_GUmut33AtoG was a gift from Alexander Green (Addgene plasmid # 139468 ; http://n2t.net/addgene:139468 ; RRID:Addgene_139468) -
For your References section:
Precise and Programmable Detection of Mutations Using Ultraspecific Riboregulators. Hong F, Ma D, Wu K, Mina LA, Luiten RC, Liu Y, Yan H, Green AA. Cell. 2020 Feb 25. pii: S0092-8674(20)30155-0. doi: 10.1016/j.cell.2020.02.011. 10.1016/j.cell.2020.02.011 PubMed 32109416
