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p_SNIPR_target_mut_36
(Plasmid #139466)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139466 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone size w/o insert (bp) 5527
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Target_mut36
  • Species
    Other
  • Insert Size (bp)
    129

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Non-cognate target for the SNIPR with a mismatch at base 36

Please note: Plasmid contains a TCG nucleotide deletion in the Target_mut_36 sequence. This mutation is not in a key region of the plasmid and should not affect its function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p_SNIPR_target_mut_36 was a gift from Alexander Green (Addgene plasmid # 139466 ; http://n2t.net/addgene:139466 ; RRID:Addgene_139466)

  • For your References section:

    Precise and Programmable Detection of Mutations Using Ultraspecific Riboregulators. Hong F, Ma D, Wu K, Mina LA, Luiten RC, Liu Y, Yan H, Green AA. Cell. 2020 Feb 25. pii: S0092-8674(20)30155-0. doi: 10.1016/j.cell.2020.02.011. 10.1016/j.cell.2020.02.011 PubMed 32109416
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