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Addgene

LentiGuide-Hygro
(Plasmid #139462)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139462 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LentiGuide-Hygro
  • Backbone manufacturer
    unpublished, Caroline Goujon
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    no sgRNA inserted; resistance gene: hygroR
  • gRNA/shRNA sequence
    no sgRNA
  • Species
    Synthetic
  • Insert Size (bp)
    2000
  • Promoter EF-1a / U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsiWI (unknown if destroyed)
  • 3′ cloning site MluI (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Generated by replacing PuroR gene with HygroR gene in LentiGuide-Puro from Feng Zhang (Addgene plasmid # 52963).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LentiGuide-Hygro was a gift from Caroline Goujon (Addgene plasmid # 139462 ; http://n2t.net/addgene:139462 ; RRID:Addgene_139462)
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