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Addgene

LentiGuide-Puro-NCOA7g1 (BB22)
(Plasmid #139457)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139457 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LentiGuide-Puro
  • Backbone manufacturer
    Feng Zhang (Addgene plasmid # 52963)
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR
  • gRNA/shRNA sequence
    g1-NCOA7 5′-ggatgtccaagggtaatctt,
  • Species
    Synthetic
  • Entrez Gene
    NCOA7 (a.k.a. ERAP140, ESNA1, NCOA7-AS, Nbla00052, Nbla10993, TLDC4, dJ187J11.3)
  • Promoter EF-1a / U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (unknown if destroyed)
  • 3′ cloning site BsmBI (unknown if destroyed)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Feng Zhang (Addgene plasmid # 52963)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LentiGuide-Puro-NCOA7g1 (BB22) was a gift from Caroline Goujon (Addgene plasmid # 139457 ; http://n2t.net/addgene:139457 ; RRID:Addgene_139457)

  • For your References section:

    The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry. Doyle T, Moncorge O, Bonaventure B, Pollpeter D, Lussignol M, Tauziet M, Apolonia L, Catanese MT, Goujon C, Malim MH. Nat Microbiol. 2018 Dec;3(12):1369-1376. doi: 10.1038/s41564-018-0273-9. Epub 2018 Nov 26. 10.1038/s41564-018-0273-9 [pii] PubMed 30478388
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