LentiGuide-Neo
(Plasmid #139449)

• Purpose: Lentiviral vector with gRNA scaffold and neomycin selectable marker
• Depositing Lab: Caroline Goujon
• Publication: Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376. doi: 10.1038/s41564-018-0273-9. Epub 2018 Nov 26.

Backbone

• Vector backbone: LentiGuide-Puro
• Backbone manufacturer: Feng Zhang (Addgene plasmid # 52963)
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: no sgRNA inserted; resistance gene: neoR
• gRNA/shRNA sequence: no sgRNA
• Species: Synthetic
• Insert Size (bp): 2000
• Promoter: EF-1a / U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsiWI (unknown if destroyed)
• 3′ cloning site: MluI (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • Lentiguide-Neo.gb
• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: Feng Zhang (Addgene plasmid # 52963).
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Generated by replacing PuroR gene with NeoR gene in LentiGuide-Puro from Feng Zhang (Addgene plasmid # 52963).