fADL-1e-flag-linker-p3
(Plasmid
#139441)
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PurposeFor cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139441 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonefADL-1e
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Modifications to backboneNcoI/NheI restriction sites for subsequent peptide cloning
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsThe F-pilus cell strain should be used for bacteriophage infection and subsequent expression
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namelinker with pelB-leader, cloning site, flag-tag and p3
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
fADL-1e-flag-linker-p3 was a gift from Alexander Gabibov (Addgene plasmid # 139441 ; http://n2t.net/addgene:139441 ; RRID:Addgene_139441) -
For your References section:
Exhaustive Search of the Receptor Ligands by the CyCLOPS (Cytometry Cell-Labeling Operable Phage Screening) Technique. Ishina IA, Filimonova IN, Zakharova MY, Ovchinnikova LA, Mamedov AE, Lomakin YA, Belogurov AA Jr. Int J Mol Sci. 2020 Aug 29;21(17). pii: ijms21176258. doi: 10.3390/ijms21176258. 10.3390/ijms21176258 PubMed 32872428
