pGEM cGdf5 (CT#688)
(Plasmid
#13944)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13944 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM-T Easy
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3015
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGdf5
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SpeciesG. gallus (chicken)
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Insert Size (bp)400
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MutationAmino acids 285-411
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Entrez GeneGDF5
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site NcoI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Partial chick Gdf 5 gene (RT-PCR product using degenerate primers, ~400bp fragment corresponding to AA 285-411 of mouse GDF5).
Addgene QC NGS analysis identifies an E193G discrepancy relative to reference sequence ASW22707.1.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEM cGdf5 (CT#688) was a gift from Cliff Tabin (Addgene plasmid # 13944 ; http://n2t.net/addgene:13944 ; RRID:Addgene_13944) -
For your References section:
Wnt-14 plays a pivotal role in inducing synovial joint formation in the developing appendicular skeleton. Hartmann C, Tabin CJ. Cell. 2001 Feb 9. 104(3):341-51. 10.1016/S0092-8674(01)00222-7 PubMed 11239392
