pGEM cCol2 (CT#617)
(Plasmid
#13943)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13943 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM-T Easy
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3015
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCollagen II
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SpeciesG. gallus (chicken)
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Insert Size (bp)434
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MutationNucleotides 182-616
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Entrez GeneCOL2A1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
434 bp amplified by RT-PCR according to nucleotides 182-616 of GENBANK sequence #M74435.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEM cCol2 (CT#617) was a gift from Cliff Tabin (Addgene plasmid # 13943 ; http://n2t.net/addgene:13943 ; RRID:Addgene_13943) -
For your References section:
Wnt-14 plays a pivotal role in inducing synovial joint formation in the developing appendicular skeleton. Hartmann C, Tabin CJ. Cell. 2001 Feb 9. 104(3):341-51. 10.1016/S0092-8674(01)00222-7 PubMed 11239392