pPEE46
(Plasmid #139425)

• Purpose: Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.
• Depositing Lab: James Fraser
• Publication: Spencer et al Fungal Genet Biol. 2020 May;138:103365. doi: 10.1016/j.fgb.2020.103365. Epub 2020 Mar 4.

Backbone

• Vector backbone: pBlueScript II SK(-)
• Backbone manufacturer: Stratagene
• Vector type: Yeast Expression
• Selectable markers: Acetamide

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mRuby3
• Alt name: Acetamidase (amdS)
• Species: Synthetic, Entacmaea quadricolor, Aspergillus nidulans
• Promoter: TEF1

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pPEE46.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains the amdS2 mRuby3 Blaster marker in the multicloning site of pBluescript II SK-. Importantly, the amdS2 mRuby3 Blaster marker can be excised using restriction enzymes XbaI and EcoRI.