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Addgene

pGWKS2
(Plasmid #139410)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139410 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBlueScript II SK(-)
  • Backbone manufacturer
    Stratagene
  • Vector type
    Yeast Expression
  • Selectable markers
    Nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP S65T
  • Alt name
    Noureothricin (NAT)
  • Species
    Synthetic, Aequorea victoria, Streptomyces noursei
  • Promoter TEF1

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains the non-codon optimised GFP S65T marker in the multicloning site of pBlueScript II SK-. Importantly, the GFP S65T marker can be excised using restriction enzymes XhoI and SpeI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGWKS2 was a gift from James Fraser (Addgene plasmid # 139410 ; http://n2t.net/addgene:139410 ; RRID:Addgene_139410)

  • For your References section:

    Broadening the spectrum of fluorescent protein tools for use in the encapsulated human fungal pathogen Cryptococcus neoformans. Spencer GWK, Chua SMH, Erpf PE, Wizrah MSI, Dyba TG, Condon ND, Fraser JA. Fungal Genet Biol. 2020 May;138:103365. doi: 10.1016/j.fgb.2020.103365. Epub 2020 Mar 4. 10.1016/j.fgb.2020.103365 PubMed 32145317
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