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pRN3P_EGFP_m6A_Tracer
(Plasmid #139403)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139403 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRN3P
  • Backbone size w/o insert (bp) 3203
  • Total vector size (bp) 4265
  • Vector type
    in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    m6A-Tracer
  • Alt name
    catalytically inactive DpnI fragment that binds methylated GATCs
  • Insert Size (bp)
    1074
  • Promoter T3
  • Tag / Fusion Protein
    • EGFP

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRN3P_EGFP_m6A_Tracer was a gift from Maria-Elena Torres-Padilla (Addgene plasmid # 139403 ; http://n2t.net/addgene:139403 ; RRID:Addgene_139403)

  • For your References section:

    Genome-lamina interactions are established de novo in the early mouse embryo. Borsos M, Perricone SM, Schauer T, Pontabry J, de Luca KL, de Vries SS, Ruiz-Morales ER, Torres-Padilla ME, Kind J. Nature. 2019 May;569(7758):729-733. doi: 10.1038/s41586-019-1233-0. Epub 2019 May 22. 10.1038/s41586-019-1233-0 PubMed 31118510
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