pRN3P_membrane_EGFP
(Plasmid
#139402)
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Purposein vitro transcription for mRNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 139402 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRN3P
- Backbone size w/o insert (bp) 3203
- Total vector size (bp) 3955
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Vector typein vitro transcription
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemembrane-EGFP
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Alt namemembrane-targeted GAP43-EGFP fusion protein
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Insert Size (bp)804
- Promoter T3
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Tag
/ Fusion Protein
- EGFP
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T3 sequence
- 3′ sequencing primer T7 sequence (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRN3P_membrane_EGFP was a gift from Maria-Elena Torres-Padilla (Addgene plasmid # 139402 ; http://n2t.net/addgene:139402 ; RRID:Addgene_139402) -
For your References section:
Genome-lamina interactions are established de novo in the early mouse embryo. Borsos M, Perricone SM, Schauer T, Pontabry J, de Luca KL, de Vries SS, Ruiz-Morales ER, Torres-Padilla ME, Kind J. Nature. 2019 May;569(7758):729-733. doi: 10.1038/s41586-019-1233-0. Epub 2019 May 22. 10.1038/s41586-019-1233-0 PubMed 31118510