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Scarlet I-FRET 10-Cerulean3
(Plasmid #139374)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139374 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Clontech C1 or N1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cerulean3 N1
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site Mfe1 (unknown if destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer CTC TAC AAA TGT GGT ATG G
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cerulean3 N1 - #54730 was used in this construct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Scarlet I-FRET 10-Cerulean3 was a gift from Richard Day (Addgene plasmid # 139374 ; http://n2t.net/addgene:139374 ; RRID:Addgene_139374)

  • For your References section:

    Direct visualization by FRET-FLIM of a putative mechanosome complex involving Src, Pyk2 and MBD2 in living MLO-Y4 cells. Day RN, Day KH, Pavalko FM. PLoS One. 2021 Dec 23;16(12):e0261660. doi: 10.1371/journal.pone.0261660. eCollection 2021. 10.1371/journal.pone.0261660 PubMed 34941939
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