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Addgene

Turquoise1-78-Venus
(Plasmid #139372)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 139372 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Turquoise1 78AA Venus
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site BglII (unknown if destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer CTC TAC AAA TGT GGT ATG G
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Turquoise -5-Venus was the base plasmid and a 78 AA linker was added.

This is a low FRET efficiency standard.

Jameson, D.M. (2016) Perspectives on Fluorescence : a Tribute to Gregorio Weber. In Springer Series on Fluorescence, Methods and Applications, , pp. 143-161.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Turquoise1-78-Venus was a gift from Richard Day (Addgene plasmid # 139372 ; http://n2t.net/addgene:139372 ; RRID:Addgene_139372)
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